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Instrumentation

 

BD FACSCanto II Flow Cytometer

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The BD FACSCanto™ II system is specifically designed for fluorescence-activated cell sorting for separation, characterization and quantification of cells in support of a range of flow cytometric applications such as immunophenotyping, cell cycle analysis and apoptosis. CIMI offers the BD FACSCanto™ II system equipped with 3 lasers (405 nm, 488 nm, 633 nm) that allow the use of up to 8 fluorochromes. The cytometer uses FACSDIVA software for acquisition and analysis.

The BD FACSCanto™ II flow cytometer is Certified for Clinical Applications.

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BD LSRFortessa Cell Analyzer

BD LSRFortessa(1)

The BD LSRFortessa™ cell analyzer is a high end analytical flow cytometer that digitizes signals 10 million times per second in 16,384 discrete levels. CIMI offers the BD LSRFortessa™ system equipped with 5 fixed alignment lasers, and up to 22 fluorchromes (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), allowing simultaneous use of all lasers. The cytometer uses FACSDIVA software for acquisition and analysis.

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Zeiss Confocal Microscopy LSM 710

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The Zeiss Confocal Microscopy LSM 710 has 6 excitation laser lines at 405, 458, 488, 514, 561 and 633nm. It is has 3 separate spectral detection channels and is capable of detecting many standard fluorophores, including DAPI, CFP, GFP, fluorescein, Rhodamine, Texas Red, RFP, CY5 and several of the AlexFluor dyes. Suitable applications include imaging of single, double and triple-labeled specimens, 3-dimensional image analysis, colocalization studies, immunofluorescence, time series and FRAP experiments. The microscope uses Zeiss LSM software for image analysis and processing.

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Germ Free Mice

CONVD NF-kB EGFP miceGerm free (GF) hosts, especially mice that have been maintained free from demonstrable microorganisms such as bacteria, viruses, fungi, and parasites throughout life, have become a powerful tool for exploring the interplay between the host and microorganisms inhabiting the human intestine. Using GF mice, investigators can demonstrate the physiological and pathological effects of the intestinal microbiota on the host. Additionally, it is possible to investigate the roles of certain important microbes by manipulating GF mice. The term gnotobiology is used to refer to these types of studies.


Germ Free Zebrafish

CONVD NF-kB EGFP fishThe advantages of zebrafish over classical vertebrate models are numerous and include high genetic and organ system homology to humans, high fecundity, external fertilization, ease of genetic manipulation, and transparency through early adulthood that enables powerful imaging modalities. Important biological processes and pathologies explored in this model include wound-healing responses, pancreatic cancer, inflammatory bowel diseases, nonalcoholic fatty liver disease, and mycobacterium infection. The ability to generate germ-free and gnotobiotic zebrafish has also led to an increasing interest in understanding cellular and molecular mechanisms of microbial-host interactions using zebrafish.